戴海峰,梁军,刘红霞,廖国清

• 1:江苏省肿瘤医院镇痛科
• 2:北京军区联勤部药品仪器检验所化学室
• 3:北京中医药大学护理学院
• 4:中国人民解放军第309医院肿瘤内科

摘要(Abstract)：

目的通过检测Hep G2及Hep G2/ADM细胞中miR-199a和MDR1/P-gp表达的差异,探讨miR-199a与肝癌化疗耐药的关系。方法利用Lipofectamine 2000转染寡核苷酸探针,荧光定量PCR方法检测miR-199a和MDR1的表达水平,Western印迹检测P-gp蛋白表达水平,MTT检测细胞存活率。结果与Hep G2细胞相比,Hep G2/ADM细胞中miR-199a表达显著下降MDR1/P-gp表达显著升高。过表达miR-199a能显著增加阿霉素对Hep G2/ADM细胞的抑制率,并抑制MDR1/P-gp的表达。干扰miR-199a能够显著地降低阿霉素对Hep G2细胞的抑制率,并增加MDR1/P-gp的表达。结论 miR-199a通过调节MDR1/P-gp的表达参与肝癌耐药形成,miR-199a可能成为逆转肝癌耐药的作用靶点。

关键词(KeyWords)： 肝癌;抗药性,MiR-199a;MDR1/P-gp;Hep G2/ADM细胞

Abstract:

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基金项目(Foundation):

作者(Author): 戴海峰,梁军,刘红霞,廖国清

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