盛望望;周秋明;王彬苏;杨宁;魏茂提;李玉明;

• 1:武警后勤学院附属医院武警部队心血管病研究所

摘要(Abstract)：

目的使用高分辨超声血流介导的血管舒张功能(flow-mediated dilation,FMD)评价高盐饮食对子痫前期大鼠内皮功能的影响,为指导子痫前期孕妇合理膳食提供科学依据。方法将18只Sprague Dawley(SD)雌性大鼠随机分为高盐饮食(high-salt diet,HS)组和正常盐饮食(normal-salt diet,NS)组,每组各9只,分别给予高盐饮食和正常盐饮食。8周后受孕,自妊娠第9天起每日在大鼠颈背部皮下注射左旋硝基精氨酸甲酯(NG-nitro-L-arginine methyl ester,L-NAME),直至妊娠第19天建立子痫前期大鼠模型。测量大鼠基线、第4周、第8周、妊娠第8天及妊娠第19天的血压和24 h尿蛋白水平。在妊娠第19天使用高分辨率小动物超声实时分子影像系统检测大鼠股动脉FMD。结果两组大鼠的终末体重无显著差异(t=-1.65,P=0.12);HS组大鼠的收缩压、平均动脉压及24 h尿蛋白水平均显著高于NS组(F_(收缩压)=96.86,P <0.01;F_(平均动脉压)=47.75,P <0.01;F_(24h尿蛋白)=56.54,P <0.01)。两组大鼠的FMD均于300 s时达到峰值,HS组大鼠股动脉FMD峰值显著低于NS组(F=175.35,P <0.01)。结论高盐饮食大鼠股动脉血管舒张功能降低,提示高盐饮食可能对子痫前期内皮功能损害更大。

关键词(KeyWords)： 高盐;子痫前期;内皮功能;血流介导的血管舒张功能

Abstract:

Keywords:

基金项目(Foundation): 天津市科技重大专项资助课题(15ZXJZSY00010);; 天津市科技计划项目(16ZXMJSY00130)

作者(Authors): 盛望望;周秋明;王彬苏;杨宁;魏茂提;李玉明;

参考文献(References)：

• [1]Huang P,Chen S,Wang Y,et al.Down-regulated CBS/H2Spath w ay is involved in high-salt-induced hypertension in Dahl rats[J].Nitric Oxide,2015,46:192-203.
• [2]Kusche-Vihrog K,Schmitz B,Brand E.Salt controls endothelial and vascular phenotype[J].Pflugers Arch,2015,467(3):499-512.
• [3]Hypertension in pregnancy.Report of the American College of Obstetricians and Gynecologists'Task Force on Hypertension in Pregnancy[J].Obstet Gynecol,2013,122(5):1122-1131.
• [4]Ghulmiyyah L,Sibai B.Maternal mortality from preeclampsia/eclampsia[J].Semin Perinatol,2012,36(1):56-59.
• [5]Paneni F,Beckman JA,Creager MA,et al.Diabetes and vascular disease:pathophysiology,clinical consequences,and medical therapy:partⅠ[J].Eur Heart J,2013,34(31):2436-2443.
• [6]Puissant C,Abraham P,Durand S,et al.[Endothelial function:role,assessment and limits][J].J Mal Vasc,2014,39(1):47-56.
• [7]Soobryan N,Murugesan S,Phoswa W,et al.The effects of silde nafil citrate on uterine angiogenic status and serum inflammatory markers in an L-NAME rat model of pre-eclampsia[J].Eur J Pharmacol,2017,795:101-107.
• [8]Shu W,Shu W,Li H,et al.Evaluation of blood vessel injury,oxidative stress and circulating inflammatory factors in an L-NAME-induced preeclampsia-like rat model[J].Exp Ther Med,2018,16(2):585-594.
• [9]Briet M,Collin C,Laurent S,et al.Endothelial function and chronic exposure to air pollution in normal male subjects[J].Hypertension,2007,50(5):970-976.
• [10]Geelhoed JJ,Fraser A,Tilling K,et al.Preeclampsia and gestat ional hypertension are associated with childhood blood press ure independently of family adiposity measures:the Avon Long itudinal Study of Parents and Children[J].Circulation,2010,122(12):1192-1199.
• [11]Chaiworapongsa T,Chaemsaithong P,Yeo L,et al.Pre-eclampsia part 1:current understanding of its pathophy siology[J].Nat Rev Nephrol,2014,10(8):466-480.
• [12]Du Pont JJ,Greaney JL,Wenner MM,et al.High dietary sodium intake impairs endothelium-dependent dilation in healthy saltresistant humans[J].J Hypertens,2013,31(3):530-536.
• [13]Cappuccio FP.Cardiovascular and other effects of salt consumption[J].Kidney Int Suppl(2011),2013,3(4):312-315.
• [14]Oberleithner H,Riethmuller C,Schillers H,et al.Plasma sodium stiffens vascular endothelium and reduces nitric oxide release[J].Proc Natl Acad Sci U S A,2007,104(41):16281-16286.
• [15]Paniagua OA,Bryant MB,Panza JA.Role of endothelial nitric oxide in shear stress-induced vasodilation of human microvasculature:diminished activity in hypertensive and hypercholesterolemic patients[J].Circulation,2001,103(13):1752-1758.
• [16]Feng W,Ying WZ,Aaron KJ,et al.Transforming growth factor-beta mediates endothelial dysfunction in rats during high salt intake[J].Am J Physiol Renal Physiol,2015,309(12):F1018-F1025.
• [17]Koleganova N,Piecha G,Ritz E,et al.Both high and low maternal salt intake in pregnancy alter kidney development in the offspring[J].Am J Physiol Renal Physiol,2011,301(2):F344-F354.
• [18]杨宁,李玉明.从多哈理论看妊娠期高血压疾病对子代心血管健康的重大影响[J].中国医学前沿杂志(电子版),2016,8(2):14-17.
• [19]李玉明,杨宁.关注生命早期心血管病风险暴露及初始预防[J].中华心血管病杂志,2017,45(4):274-276.